Sequence based genotyping (SBG, GBS)

KeyGene reference: P048, P053 & P060

Invention

The invention entails combining steps of complexity reduction with the introduction of identifiers through ligation or amplification and next-generation sequencing, which allows for highly efficient multiplexed genotyping. Apart from steps of library preparation, the patents cover proprietary elements such as a barcoded 3’-T-overhang adapter and library preparation kits for multiplex genetic variation detection comprising ligation adapters and amplification primers.

Benefit

The patented technology is the first of its kind to genotype SNP, SSR and indel polymorphisms in a single streamlined process for multiple samples using SBG. Prior knowledge of a reference genome sequence is not required.

Together with the patent family on KeyPoint^®, KeyGene’s global SBG portfolio revolutionized the discovery and detection of polymorphisms.

The various workflows, kits, and proprietary elements covered by the patents have wide application in medical and agricultural research and diagnostics, ranging from scoring Single Nucleotide Polymorphism (SNP) markers linked to valuable traits in crops and animals to detection of specific mutations associated with human diseases. Examples of such assay methods are described in Truong et al.^1, and Poecke et al.², including related methods such as GBS³, two-enzyme GBS⁴, tunable GBS⁵, RAD⁶, ddRAD⁷, 2b-RAD⁸, SLAF-seq⁹, and hybrid-capture based NGS¹⁰.

Single-fee license for SBG use in research

KeyGene understands the value of Sequence Based Genotyping for research. We very much welcome the use in research. Therefore we have created an easy, low-cost and transparent solution for non-profit research organizations to easily access KeyGene’s Sequence-Based Genotyping technology for internal research.
Are you interested in a research or commercial license, please contact our licensing team.

Patents

Click on the links below for an overview of the patent families in the European Patent Register:
WO2006/137733
WO2007/073165
WO2007/114693
Please note that the European Patent Register may not be extensive and/or accurate, for which KeyGene is not responsible. Please contact your patent expert for further information.

Would you like to explore the possibilities for obtaining a license for Variation detection by Next Generation Sequencing? You can contact our licensing team by mail.

Would you like to know more about our technologies and licensing possibilities? Visit our webpage about Licensing KeyGene’s proprietary technologies.

1. Truong et al., PLoS ONE. 2012;7(5):e37565.
2. Van Poecke et al., Plant Biotechnol J. 2013 Sep;11(7):809-817.
3. Elshire et al., PLoS ONE 2011; 6(5): e19379.
4. Poland et al., PLoS One 2012; 7(2): e32253.
5. Ott et al., Nucleic Acids Research 2017; 45 (21)
6. Baird et al., PLoS ONE 2008; 3(10): e3376.
7. Peterson et al., PLoS ONE 2012; 7(5): e37135.
8. Wang et al., Nat Methods. 2012; 9(8):808-810.
9. Sun et al., PLoS ONE 2013; 8(3): e58700.
10. Mamanova et al., Nature Methods 2010; 7(2) 111–118