SNPSelect

KeyGene reference: P063, P134 & P213

Invention

Provided are innovative and proprietary oligoligation formats that are collectively indicated by the name KeyGene® SNPSelect.

Benefits

SNPSelect offers flexible genotyping in which the number of SNPs and number of samples can easily be varied. Also adding extra SNPs to an assay is possible.

SNPSelect can be used to genotype SNPs from a low multiplex format up to genotyping of thousands of SNPs simultaneously. Using SNPSelect, genotyping of polyploids is feasible with high concordancy rates. Furthermore, the design of the probes, as well as the scoring of the genotypes, is automated.

Together with KeyGene’s invention on probe amplification, a SNPSelect approach allows for a very cost-effective genotyping method.

Vision

The invention results in a more accurate calling of the genotypes as a single error in base calling will not result in wrong allele calling. Additionally, when using the UMI approach, genotyping of polyploid samples is extremely accurate and allows for the quantitative assessment of the presence of specific alleles, while maintaining flexibility in the number of samples and the number of SNPs. Using a mixture of uniquely tagged oligo ligation probes, effective genotyping of 326 SNPs was proven in tetraploid genomic samples in a KeyGene^® SNPSelect assay¹.

The technology results in flexibility from low to high SNP numbers in combination with a low to high number of samples. Other technologies are either low or highly multiplexed. This implicates that when using other techniques, at least 2 different technologies are needed to do the work. SNPSelect has the capabilities to use one single technology to use SNPs in a low multiplex context up to a very high multiplex setting.

Patents

Click on these links for an overview of the patent families in the European Patent Register: WO2007/100243, WO2013/009175, and WO2020/169830

Please note that the European Patent Register may not be extensive and/or accurate, for which KeyGene is not responsible. Please contact your patent expert for further information.

Scientific paper describing SNPSelect

1. Hogers RCJ, de Ruiter M, Huvenaars KHJ, van der Poel H, Janssen A, van Eijk MJT, et al. (2018) SNPSelect: A scalable and flexible targeted sequence-based genotyping solution. PLoS ONE 13(10): e0205577.

Would you like to explore the possibilities for obtaining a license for SNPSelect? You can contact our licensing team by mail.

Would you like to know more about our technologies and licensing possibilities? Visit our webpage about Licensing KeyGene’s proprietary technologies.